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To determine whether extracellular neurotransmitters could influence lactate production from pyruvate, the effects of N-methyl-D-aspartate (NMDA), an excitatory transmitter, on lactate production by cultured cortical astrocytes were investigated. Lactate production was measured using [1-14C]pyruvate as substrate in the presence of beta-NAD+, nicotinamide, and rotenone. NMDA, 50 micromol/l, concentration-dependently inhibited lactate production by cortical astrocytes. The inhibitory effect was blocked by tetrodotoxin, indicating that the effect was mediated via presynaptic non-NMDA receptors. The incubation of these cells with 1 mmol/l NMDA, concentration that activated non-NMDA receptors but which did not affect lactate production, also inhibited lactate production. The effect was attenuated by 6-cyano-7-nitro-quinoxaline-2,3-dione. The inhibition was also produced by D,L-2-amino-5-phosphonopentanoic acid, an N-methyl-D-aspartate receptor antagonist, and by kainate. The results suggest that NMDA inhibits lactate production from pyruvate in cultured astrocytes, probably via non-NMDA receptors on the pre-synaptic terminals.In response to the recent demand from National Parks Foundation for me to do a “Travels with Liz” story, it’s with great pleasure I speak to you from my parlor room in Taos, New Mexico.
I first met Liz about six years ago when a car I was driving was in a horrific three-car accident. She had picked me up from the hospital and was dutifully waiting as I recovered.
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The $1 million, 10,500-square-foot fence, with vertical wooden posts spaced about 50 feet apart, was installed overnight with help from several King’s Bluff homeowners who volunteered to assist, said Jeff Janes, who represents the King’s Bluff Neighborhood Association.
The fence was installed as part of the restoration of a historic building started in 2003 that was almost completely gutted by vandals, Janes said.
“It’s been a real sore spot for the community for a while,” he said. “The church spent literally thousands of dollars on that property trying to get it back, and every time they start to make some progress, they get vandalized again.”
The church has put an estimated $15,000 into the repairs so far.
Previous attempts to improve the neglected building and grounds included more chain-link fencing and several high-powered lights set into the lot, Janes said. Each effort was ultimately abandoned after complaints of neighborhood safety and quality of life concerns.
“Unfortunately, we’ve had issues before where residents just kept putting up these lights and making noise,” he said. “It’s just the way it is in the neighborhood. You’ve just got to make that choice, and the church is willing to do that.”
Because of the massive care that has gone into the renovation work, the church isn’t willing to turn the building over to an as-of-yet-unknown buyer, said Andy